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[Article Of Journal] 2014 JOE : Comparison of the Biological Properties of ProRoot MTA, Ortho MTA, and Endocem MTA Cements

Author
MARUCHI
Date
2017-11-27 13:59
Views
338
Abstract
Introduction : Ortho MTA(BioMTA,Seoul,Korea) and Endocem MTA (Maruchi,Wonju-si,Korea) were recently
developed to overcome the disadvantages of ProRoot MTA(Dentsply,Tulsa,OK). This study aimed to compare
the biological properties of Ortho MTA and Endocem MTA with those of ProRoot MTA using the preosteoblast like cellline MC3T3-E1.
Methods
The setting times of calcium silicate – based cements (CSCs) and their effects on the pH of distilled water during storage were determined according to ISO standards. MC3T3-E1
cells were cultured with ProRoot MTA, Ortho MTA, and Endocem MTA. The viability of the cells was assessed using the Cell Counting Kit-8 assay (DojindoLaboratory,Kumamoto,Japan) on the super natants of CSCs, and the cells’osteopontin production was determined by an enzyme - linked immunos or bentassayna culture with
the materials on days 3 and 7 of incubation.
Results
Endocem MTA exhibited asign ifcantly shorter setting time (15.30.5 minutes) than did ProRoot MTA and Ortho MTA (318.056.0 and 324.32.1 minutes, P<.05) . Additionally, all CSCs caused their storage water to be come highly alkaline after 7days. Ortho MTA was sign icantly more cytotoxic than ProRoot and Endocem MTA (P<.05) . ProRoot MTA induced signicantly more OPN production than Ortho MTA and Endocem MTA on both days 3 and 7(P<.05).
Conclusions
ProRoot MTA appeared to be superior to Ortho MTA and Endocem MTA in terms of biological properties although
EndocemMTAexhibitedtheshortestsettingtimeand
presentedlowercytotoxicitywithosteoblastlikecells.