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[Article Of Journal] 2013 JOE : Biological Effects and Washout Resistance of a Newly Developed Fast-setting Pozzolan Cement
Biological Effects and Washout Resistance of a Newly Developed Fast-setting Pozzolan Cement
Yoorina Choi, DDS, Su-Jung Park, DDS, MSD, Seoung-Hoon Lee, PhD, Yun-Chan Hwang, DDS, PhD, Mi-Kyung Yu, DDS, PhD,and Kyung-San Min, DDS, PhD
Introduction: Mineral trioxide aggregate has been widely used as a retrograde filling material. The aim of this study was to evaluate the biological effects of a newly developed fast-setting, mineral trioxide aggregate–derived pozzolan cement (Endocem). Furthermore,we explored whether this cement is resistant to washout in comparison with ProRoot.
Methods: Biocompatibility was evaluated on the basis of cell morphology and a viability test. The __EXPRESSION__ of osteogenic genes was evaluated by performing real-time polymerase chain reaction, and calcified nodule formation was assessed by alizarin red S staining. The setting time was measured, and washout testing was performed by placing the material into fetal bovine serum.
Results: The biocompatibility and osteogenicity of Endocem were similar to those of ProRoot. Moreover, Endocem
showed a higher resistance to washout than ProRoot did.
Conclusions: These results suggest that Endocem can be used as an available retrograde filling material because it sets faster and shows similar biological effects when compared with ProRoot.
(J Endod 2013;39:467–472)